The Effect of 17% Ethylenediaminetetraacetic Acid and 10% Citric Acid Solutions with Manual and Ultrasonic Agitation on Smear Layer Removal and Intraradicular Dentine Erosion: A Scanning Electron Microscope Evaluation The Effect of 17% Ethylenediaminetetraacetic Acid and 10% Citric Acid Solutions with Manual and Ultrasonic Agitation on Smear Layer Removal and Intraradicular Dentine Erosion: A Scanning Electron Microscope Evaluation

Main Article Content

Alessandro Rodrigo Maggioni
Helena Rosa Campos Rabang
Brenda Paula Figueiredo de Almeida Gomes
Luiza Gonçalves Roma
Isabel Coelho Gomes Camões

Abstract

Este estudo ex vivo avaliou a eficácia de diferentes protocolos de irrigação final na remoção da smear layer e na erosão da dentina intrarradicular.Trinta e cinco caninos humanos extraídos foram ins- trumentados e divididos aleatoriamente, de acordo com as técnicas de irrigação final utilizadas, em 7 grupos: 1 (ED3M) e 3 (ED3US), EDTA a 17% por 3 minutos com agitação manual e ultrassônica, respectivamente; 2 (CA30M) e 4 (CA30US), ácido cítrico a 10% por 30 segundos com agitação manual e ultrassônica, respectiva- mente; 5 (CA3M) e 6 (CA3US), ácido cítrico a 10% por 3 minutos com agitação manual e ultrassônica, respectivamente; e 7 (Na3), NaOCl a 5,25% por 3 minutos sem agitação (controle). Todas as amostras foram irrigadas com NaOCl a 5,25%, divididas longitudi- nalmente e examinadas em microscopia eletrônica de varredura (MEV) nos terços apical, médio e coronal. Os dados foram analisa- dos pelos testes de Kruskal-Wallis e Mann-Whitney. A eficácia do EDTA 17% e do ácido cítrico 10% na remoção da smear layer foi significativamente maior que NaOCl 5,25% (controle). Não houve diferenças significativas entre os protocolos finais de irrigação na remoção da smear layer ou nos efeitos erosivos. Entretanto, ao comparar os terços dos grupos 1 (ED3M) e 2 (CA30M), a menor remoção da camada de smear layer e erosão foram observadas no terço apical, mas o procedimento do grupo 4 (CA30US) foi mais eficaz que 1 (ED3M) (p = 0,0004), 2 (CA30M) (p = 0,0018) ou 3(ED3US) (p = 0,0003) na remoção da smear layer no terço apical.Concluiu-se que os protocolos utilizados neste estudo foram se- melhantes na remoção da smear layer e efeitos erosivos.

Article Details

How to Cite
Rodrigo Maggioni, A., Rosa Campos Rabang, H., Paula Figueiredo de Almeida Gomes, B., Gonçalves Roma, L., & Coelho Gomes Camões, I. (2020). The Effect of 17% Ethylenediaminetetraacetic Acid and 10% Citric Acid Solutions with Manual and Ultrasonic Agitation on Smear Layer Removal and Intraradicular Dentine Erosion: A Scanning Electron Microscope Evaluation. Naval Dental Jounal, 47(1), 7-13. https://doi.org/10.29327/25149.47.1-2
Section
Original Article
Author Biographies

Alessandro Rodrigo Maggioni, Brazilian Navy, Central Navy Dental Clinic

DDS, Dental Department, Endodontic clinic, Naval Dental Center, Brazilian Navy, Rio de Janeiro, Brazil.

Helena Rosa Campos Rabang, Department of Education, Endodontics, Naval Dental Center, Brazilian Navy.

DDS, MSc, D; Department of Education, Endodontics, Naval Dental Center, Brazilian Navy,

Captain of sea and war former director of the Central Odontoclínica da Marinha Rio de Janeiro, Brazil

Brenda Paula Figueiredo de Almeida Gomes, Piracicaba Dental School, State University of Campinas

DDS, MSc, D. Department of Restorative Dentistry, Endodontic Division, Piracicaba Dental School, State University of Campinas, Piracicaba-SP, Brazil

Luiza Gonçalves Roma, Endodontic clinic, Naval Dental Center, Brazilian Navy

DDS, Dental Department, Endodontic clinic, Naval Dental Center, Brazilian Navy, Rio de Janeiro, Brazil

Isabel Coelho Gomes Camões, Department of Dental Clinics, Endodontic Division, Dental School, Fluminense Federal University

DDS, MSc, D. Department of Dental Clinics, Endodontic Division, Dental School, Fluminense Federal University, Niterói-RJ, Brazil

References

1. McComb D, Smith DC. A preliminary scanning electron microscopic study of root canals after endodontic procedures. J Endod. 1975 Jul 1(7):238-42.
2. Goldman LB, Goldman M, Kronman JH, Lin PS. The efficacy

of several irrigating solutions for endodontics: a scanning electron microscopic study. Oral Surg Oral Med Oral Pathol. 1981 Aug 52(2):197-204.
3. Mader CL, Baumgartner JC, Peters DD. Scanning electron microscopic investigation of the smeared layer on root canal walls. J Endod. 1984 Oct 10(10):477-83.
4. Virdee SS, Seymour DW, Farnell D, Bhamra G, Bhakta S. Efficacy of irrigant activation techniques in removing intracanal smear layer and debris from mature permanent teeth: a systematic review and meta-analysis. Int Endod J. 2018 Jun 51(6):605-621.
5. Czonstkowsky M, Wilson EG, Holstein FA. The smear layer in endodontics. Dent Clin North Am. 1990 Jan 34(1):13-25.
6. Cobankara FK, Adanr N, Belli S. Evaluation of the influence of smear layer on the apical and coronal sealing ability of two sealers. J Endod. 2004 Jun 30(6):406-9.
7. White RR, Goldman M, Lin PS.The influence of the smeared layer upon dentinal tubule penetration by plastic filling materials. J Endod. 1984 Dec 10(12):558-62.
8. Byström A, Sundqvist G. Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy. Scand J Dent Res. 1981 Aug 89(4):321-8.
9. Bystrom A, Sundqvist G. The antibacterial action of sodium hypochlorite and EDTA in 60 cases of endodontic therapy. Int Endod J. 1985 Jan 18(1):35-40.
10. Sen BH, Wesselink PR, Türkün M. The smear layer: a phenomenon in root canal therapy. Int Endod J. 1995 May 28(3):141-8.
11. Hülsmann M, Heckendorff M, Lennon A. Chelating agents in root canal treatment: mode of action and indications for their use. Int Endod J. 2003 Dec 36(12):810-30.
12. De-Deus G, Paciornik S, Mauricio MH. Evaluation of the effect of EDTA, EDTAC and citric acid on the microhardness of root dentine. Int Endod J. 2006 May 39(5):401-7.
13. Sceiza MF, Daniel RL, Santos EM, Jaeger MM. Cytotoxic effects of 10% citric acid and EDTA-T used as root canal irrigants: an in vitro analysis. J Endod. 2001 Dec 27(12):741-3.
14. De-Deus G, Paciornik S, Pinho Mauricio MH, Prioli R. Real-time atomic force microscopy of root dentine during demineralization when subjected to chelating agents. Int Endod J. 2006 Sep 39(9):683-92.
15. Deari S, Mohn D, Zehnder M. Dentine decalcification and smear layer removal by different ethylenediaminetetraacetic acid and 1-hydroxyethane-1,1-diphosphonic acid species. Int Endod J. 2019 Feb 52(2):237-243.
16. Yamada RS, Armas A, Goldman M, Lin PS. A scanning electron microscopic comparison of a high volume final flush with several irrigating solutions: Part 3. J Endod. 1983 Apr 9(4):137-42.
17. Zehnder M. Root canal irrigants. J Endod. 2006 May 32(5):389-98.